Simplify the switch to hilic with raptor hilicsi columns. The rugged beh particles wide usable ph range overcomes this ph limitation and results in long column lifetimes. Realkalization of the reinforcing steel to stop corrosion. If the column performance is poor after regenerating and cleaning, call your local waters office for additional support. Hydrophilic interaction liquid chromatography columns strongly retain compounds not retained under conventional reversed phase conditions, without the need. Hydrophilic interaction chromatography, hilic, chromatographic characterization, structural selectivity, ion exchange interactions abstract. The raptor hilicsi column simpli fies the switch to hilic because it delivers rugged raptor performance.
The ace hilicn column is a bonded neutral character phase. By using an amide or amino bonded phase column, polar compounds can be retained by a normal phase or hydrophilic interaction chromatography retention mechanism. Quickly and easily reversed hplc column develop methods based on compound type, usp methods, application, or desired column phase. It seems to me that waters has the best service and help for users in europe. Thermo fisher scientific understands the demands of chemical analysis in the pharmaceutical industry. Chromatographic characterization of stationary phases for. Thermo scientific acclaim hilic10 product manual pdf. Storage of hilic columns with buffer is recommended to decrease equilibration time. Cmms software helps you quickly address maintenance requests, improve facility conditions through routine maintenance, and manage multiple buildings with mobile work orders. The attached manual describes the acquity uplc beh columns and includes user recommendations. So take off you lab coat, relax and let our web tool do the work. Supelco ondemand hplc and uhplc webinars and videos on theory, fundamentals.
This article addresses common problems that analysts encounter when running hilic methods and details how to avoid them and successfully use the technique. Figure 1 shows a uv chromatogram with an overlay of five. Hydrophilic interaction chromatography hilic is therefore. Atlantis hilic silica columns are used in combination with high organic mobile phases 80% acetonitrile to provide retention of analytes that are simply too polar to be retained by reversephase chromatography. How to avoid common problems with hilic methods restek. Atlantis hilic silica columns are designed to retain very polar, organic. Based on ultrapure silica gel zwitterionic hilic phases are ideal for the analysis of polar and ionizable compounds. Important goals in hplc are increasing speed and maintaining resolution. Agilent infinitylab solutions for hplc and uhplc include instruments, columns, and supplies to help your lab achieve. Continuous use of hplc columns under high pressure can cause deterioration and overload of the equipment. Storing a column with buffer in the mobile phase is typically not recommended for a number of reasons, most have little to do with sorbent stability.
Results and discussion the separation of a tryptic digest of infliximab was obtained with a 45 minute gradient and a total analysis time of 75 minutes, including column wash with high buffer content and reequilibration at initial conditions. I want to test my hilic column with different mobile phase %. Identifying the precise location of glycans within a protein structure is essential to delivering safe and effective. Shimpack velox hilic shimadzu shimadzu corporation. Application of hilic column for the determination of catechins. All of these situations are likely to result in your instrument going offline for maintenance. If you do not find a reliable solution there, i recommend to use spe as the sample cleanup tool. Hydrophilic interaction chromatography hilic was first introduced by alpert in 1990 and since then has attracted increasing attention. Ensure the best results from your chromatography analysis with our highperformance columns.
New agilent infinitylab poroshell 120 hilic chemistries combine innovative particle technology and bonded phases to improve retention and peak shape for polar analytes. Maintenance teams help provide a safe and stable learning environment for students, teachers, and other staff. Hilic stationary phases thermo fisher scientific us. An effective method development approach uses both an xbridge hilic column and an xbridge reversedphase column to screen. Multi mode column for rp, np, hilic and ic, aminopropyl modification without endcapping, outstanding mechanical and chemical stability. Atlantis hilic silica columns are designed to retain very polar, organic molecules that are too polar to retain by rp. Maintenance of spray stability is a key challenge in lcms analysis of. Hydrophilic interaction chromatography or hydrophilic interaction liquid chromatography, hilic is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. Hilic stands for hydrophilic interaction liquid chromatography. Agilent zorbax hilic plus hplc columns data sheet column characteristics a typical quality control qc chromatogram for a 4.
Our separation and detection technologies, combined with experience and applications competence, provide. Agilent infinitylab poroshell 120 columns family agilent. Agilent infinitylab poroshell 120 columns for hplc and uhplc more than 15 years ago, agilent introduced poroshell 300, the first commercial columns to use superficially porous particle technology. Acquity uplc beh hilic columns offer advantages such as retention and separation of very polar basic compounds, alternate selectivity as compared. The hamilton prpc18 is a new hplc column designed to provide highefficiency, reversedphase separations over an extended column life in nearly any mobile phase or ph. Waters new hilicbased glycoprotein column enhances glycan. Whether youve run hilic methods before or are attempting to for the first time. Understanding the possible hilic retention mechanisms involved for a particular columnsolventanalyte system is crucial in the process of selecting the right hilic stationary phase, leading to a successful application. This particle design exhibits very high column efficiency due to the. The perkinelmer elite 608 columns specifically designed for the analysis of semivolatile pesticides. So you can quickly and efficiently separate challenging, highly charged polar compoundsand achieve superior analytical sensitivity in ms mode using msfriendly eluents. The acclaim mixed mode hilic1 columns stationary phase consists of a hydrophobic alkyl chain with a terminal diol group.
This is particularly true for gags, because the polarity of a given oligosaccharide increases with increasing length and sulfate content. Oppositely, a plain silica column will act as a cation exchanger when ph is raised above 4, as the oh groups forms. Amino phase based on ultrapure silica gel purity 99 %, metal content waters. Methods of concrete column repair for cracks and damages. Care, maintenance, and troubleshooting oftroubleshooting. Hilic an alternative separation technique for glycopeptides.
This section provides some general guidelines for the care, use and storage of hilic columns. Coming to the actual stability of the column, hilic columns may degrade in high. Column troubleshooting is more usually performed by reference to the chromatogram and chromatographic peak. Hilic and aqueous normal phase chromatography the terms hydrophilic interaction liquid chromatography hilic and aqueous normal phase anp. Consider an ionexchange method for the sample cleanup using a mixedmode ionexchanger, followed by elution in high organic with injection onto the hilic column. Choose from our wide selection of hplc and lc columns as well as gc columns for both your perkinelmer and non perkinelmer chromatography instrumentation. Care, maintenance, and troubleshooting oftroubleshooting of hplc columns columns and consumables edward kim apppp glications engineer january 17, 2008. Find raptor reliability modeling software related suppliers, manufacturers, products and specifications on globalspec a trusted source of raptor reliability modeling software information. The stationary phases used in hilic are quite diverse and users often have the misconception that hilic columns are interchangeable. Encasement or enlargement of the column cross section jacketing. Hydrophilic interaction chromatography hilic is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. Learn how high purity silica of this hplc column is used to retain and separate small, polar analytes specifically in hydrophilic interaction chromatography. A robust hplc uhplc hilic column with neutral character. If this flushing procedure does not solve the problem, purge the column with 5.
Hilic is an increasingly popular alternative to reversedphase lc for challenging polar analytes because it provides better retention of watersoluble. Unlike the highly aqueous mobile phases required for polar retention in rp separations, atlantis hilic silica columns employ highly volatile 80% organic mobile phases which are ideal for mass spectrometry ms response and sensitivity. This mode of hplc is now commonly referred to as hilic, hydrophilic interaction liquid chromatography. Prior to storage, buffered solutions used during hilic analysis should be washed out of the column and the hplc system. Now, infinitylab poroshell 120 columns have become critical tools for solving the latest challenges in the analytical world. Triazole column is an unique hilic phase that has cyclic amino groups. The qc test with the performance of your column is shown on the column performance report enclosed with your column. Hilic uses hydrophilic stationary phases with reversedphase type eluents. Whether youve run hilic methods before or are attempting to for the first time, there are significant differences between reversedphase and hilic methods that must be considered. The hilicn stationary phase is based upon the ultrapure ace silica platform with a proprietary polyhydroxy bonded functionality.
View the large range of highly specialized, high performance hplc columns designed for the analysis of small molecules. This unique bonded phase provides a highly polar stationary phase on which to perform hilic. Hilic column general information halo description halo hilic is a highspeed, highperformance liquid chromatography column based on a new fusedcoretm particle design. Eliminating ionpairing reagents improves detection limits, method reproducibility and robustness, while reducing instrument maintenance due to harsh mobilephase environments.
Enhanced usability, traceability, and security with a preprogrammed id tag. Uhplc guard columns to further extend the life of your analytical column. Chromatography and mass spectrometry training, subjects covered include, hplc, gc, spe, mass spec, hilic. Cathodic protection to stop reinforcing steel corrosion. Not a good type of mode hilic is a mode of chromatography, not a column. Optimizing hilicbased analyses of rapifluorms labeled sialylated nglycans. Reduced need for sample preparation due to 2 m frits on 2. Improved hilic lcms of heparinoids using a chip with postcolumn makeup flow. I am using a zichilic column to detect polar molecules, but i have some background peaks. Hilic seems to be a missing tool in the analysis of polar and ionized compounds, which are too strongly retained on polar stationary phases used in normal phase mode andor are poorly separated when reserved phase mode is. Methods of concrete column repair for damages and cracks columns may be repaired by using one or more of the following methods. Does anyone know why the hilic column stability is affected by the.
Note if above treatment fails to revive the column, the column should be replaced. A suitable wash solution is 60% organic solvent in water unless otherwise specificed in product manuals. Thermo scientific product manual for acclaim hilic10 columns page of 23 06533902. General purpose bare silica column for normal phase and hilic applications. Cation exchange hilic polysulfoethyl a similar to anion exchange hilic, the proper adjustment of ph determines the mode of operation. Optimizing hilicbased analyses of rapifluorms labeled. Specific advice can be found in the appropriate product manual. Hplc software and lc drivers for powerful and flexible instrument control. Our reversed phase hplc column match web tool allows you to develop reversed methods without having to go on the lab. Hilic columns should be stored under hilic conditions. Zwitterion hilic mnhilic nucleodur hilic is a special zwitterionic modified stationary phase based on ultra spherical nucleodur particles.